After transport to Belgium, colonies
were further purified through successive transfers in and plating on
the appropriate media, and a catalase test was performed. Potential
LAB (catalase-negative) and AAB (catalase-positive) isolates were
grown in MRS and mannitoleyeast extractepeptone (MYP)
medium [2.5% D-mannitol, 0.5% yeast extract, and 0.3% bacteriological
peptone (Oxoid); wt/vol], respectively, and stored at 80 C.