4. Results and discussions
4.1. Membrane functionalization
In this work, the surface of commercial regenerate cellulose membrane was functionalized before immobilizing biomolecules through a three steps reaction:
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oxidation reaction with a NaIO4 solution (RC-OX);
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spacer introduction (EDA), resulting in a Shiff base formation (RC-OX-EDA);
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activation by GA (RC-OX-EDA-GA).