ConclusionThe present study established a low-cost purification of milkwhey-derived nisin by HIC. We obtained a high degree purifi-cation of nisin without protein precipitation, which allowsstep/cost reduction. It was possible to recover nisin with wateror PBS, which allows more possible applications of nisin.Water was the best eluent, with an increase of up to 775-fold in the antimicrobial activity. As per the outcomes of thiswork, milk whey probably contains factors, as remains ofprecipitated proteins, derived from the nisin production pro-cess, that decrease its activity, although high activity valueswere obtained in the crude extract. These factors were eas-ily removed by HIC purification, evidenced by an even higherantimicrobial activity.We conclude that production of nisin using milk whey asculture medium and HIC in the downstream process is cost-effective and results in a highly purified nisin product.