During the fermentation process, yeast cells in sake mash proliferate and begin to produce ethanol. During the late fermentation stage, yeast cells begin to die in response to the increasing ethanol concentration and subsequent yeast cell lysis occurs along with the leakage of their cellular contents. This phenomenon is indicated by an increase in amino acid nitrogen in sake (7) or S-adenosylmethionine leakage into sake mash (8). The amount of amino acid nitrogen and zinc in sake, which is an indicator of cellular content leakage into sake mash (9), revealed a positive correlation with hineka after storage 10 and 11. However, no studies have examined the relationship between yeast cell lysis in sake mash and the formation of DMTS, which is the main component of hineka in sake after storage.