2.3. Antibacterial assay
2.3.1. Agar well diffusion method
One hundred microliters of inoculum, equivalent to 107 CFU/mL,
was mixed with 20 mL of warm melted BHA. The mixture was then
poured into the plate with a 6 mm diameter metal cup. After solidifying
of BHA, the metal cups were removed and the well was
added with 100 mL of each plant extract. The plate was incubated at
37 C for 24 h. A 10% DMSO were taken as a negative control. The
antimicrobial activity of each plant extract was determined by
measuring the diameter of the zone of the inhibition in millimeters.
Duplicates were maintained and the experiment was repeated
thrice.