Aims: To construct a stable and functional lactic acid bacteria community which can be
used in the fermentation of bamboo-shoot shell to improve the tasty and flavor.
Methodology: Using naturally fermented bamboo-shoot shell as initial sample, a qualitative
microorganism source had been prepared by the technique of continuous restricted
subcultivation. Then some colonies producing acid were selected by plate isolation and
streaking from above source. Morphology & phenotype experiments, catalase test, GC-MS
and 16S rRNA analysis were employed to identify the isolates. Finally, the ensilage
experiment of moist bamboo-shoot shell was carried out to assess the potency of the
selected lactic acid bacteria.
Results: After a serial of subcultivation, a stable and functional bacteria community
producing acid had been constructed in the 23rd culture of bamboo-shoot shell. The bacteria
community consisted of Lactobacillus plantarum subsp plantarum, Lactobacillus plantarum,
Lactobacillus sp T2R2C12 and Lactobacillus pentosus. Inoculated with the lactic acid
strains, the culture pH of bamboo-shoot shell decreased sharply, meanwhile, the
accumulation of lactic acid amounted to 4.01% at the end of ensiling.
Conclusion: Continuous restricted subcultivation is an effective technique to construct lactic
acid bacterial community. We isolated several strains of lactic acid bacterial successfully
from naturally fermented bamboo-shoot shell. The strains can participate in the fermentation
of forage made of bamboo-shoot shell.