Total phenolic content: Total phenolic contents of extract were determined by using Folin-Ciocalteu reagent and external calibration with pyrogallol. Briefly, 100 μL of extract (1 % w/v) was mixed with 4.5 mL of aquadest then added with 100 μL of Folin-Ciocalteau reagent and the reagents were mixed throughly. After three minutes 300 μL of Na2CO3 was added and then the mixtures were allowed to stand in room temperature for 2 h. The absorbance was measured at 760 nm using a HITACHI, U-2000 spectrophotometer. The estimation of total phenolic in the extract was determined as equivalent mg g-1 of pyrogallol through an equation obtained from pyrogallol calibration curve (y = 2.5957x +0.0784, R2 = 0.9857).