Description
CCD Modified Agar is formulated according to the ISO Standard 10272-
1:2006 and is intended to detect and enumerate Campylobacter spp from
food and animal feeding stuffs.
After determining that campylobacter species grow best on solidified
Nutrient Broth No. 2 compared to other media workers (1983) carried
out a systematic survey of alternatives to blood for neutralizing oxygen
toxicity. A combination of 0,4% charcoal, 0,25% ferrous sulfate and
0,25% sodium pyruvate proved best.
A further study surveyed the suppressive effect of several inhibitors on the
undesirable microbiota showing deoxycholate and cefazolin as the most
effective inhibitory agents. Later, in 1984, Hutchinson and Bolton replaced
cefazolin (10 mg/L) with cefoperazone (32 mg/L). This allowed fewer
contaminants to grow, and permitted the modified medium (modified
CCD Agar or mCCDA) to be used at 37ºC. However amphotericin B was