Fig. 1. C. reinhardtii cw15-325 growth at different temperatures. (A). Cell number. Cells were grown in TAP medium containing 7.5 mM ammonium and 1 g acetate. Cell numberwas analyzed at the indicated times for cultures as they grew continuously in the light at 22◦C, 14◦C or 7◦C. Each data point represents the average of three independentexperiments (±SE). Significant differences with respect to the 7◦C are indicated with *. (B) Fresh weight (FW) and protein content. Cells were grown as described in legendto (A). Insert shows cells incubated at 7◦C. Each data point represents the average of three independent experiments (±SE). (C). DIC micrographs of cells incubated at 7◦C.The images shown are representative of typical cells at the different time points. Scale bar equals 10 m.