2.3. Experimental design
The experiments had a completely randomized factorial design
(3 3). A total of 27 bags were prepared, three per treatment and
High pressure processing (HPP) constitutes a promising alternative
to substitute thermal treatments like blanching and
pasteurization, taken into account that it can be applied at room
temperature. Since beneficial compounds such as vitamins and
volatiles remain virtually unaffected, the original flavor and nutritional
quality of foods are suitably preserved. Among the limitations
to be overcome, it can be mentioned the difficulty of
inactivating bacterial spores and some enzymes such as PPO. Since
this enzyme is strongly linked to enzymatic browning, its baroresistance
poses a particular difficulty for the successful application
of HPP in fruits.