Northern blot analysis was performed to determine
the pattern of StCyP gene expression in
tissues of healthy potato plants. For this total
RNA was extracted from young leaves, mature
leaves, stems, tuber buds and tubers. After hybridizing
with the StCyP cDNA probe, a single
transcript of approximately 1 kb was detected in
all the tissues assayed here (Fig. 4, upper panel).
This analysis revealed that StCyP mRNA accumulated
at very high levels in photosynthetic organs,
such as leaves and stems, and in developing or