The inoculum for further enzyme modulation and other
studies was prepared using Luria Broth (LB) medium. The
fresh overnight culture was used as an inoculum for the production
of amylase.10 The inoculated medium was incubated
at 37 C for 48 h by shake flask fermentation method at
200 rpm. The culture broth was then centrifuge at 8000 g
10 min at 4 C. The free cell supernatant was used as an
extracellular crude enzyme