Wheat grain spawn of P. sajor-caju was obtained from National Agriculture Research Council (NARC), Khumaltar,
Lalitpur, Nepal. Three substrates namely maize stalk, pea residue (tendrils) and banana leaves including two
supplements viz. rice bran and chicken manure were used as ingredients for experiment. The agricultural residues
such as pea residue (tendrils) and maize stalk were collected form Kirtipur, Kathmandu and banana leaves
were obtained from Siraha district (tropical region) of eastern Tarai of Nepal. Rice bran was collected from local
rice mill in Kirtipur and chicken manure was obtained from private livestock farm from the same area. A total of nine
treatments, three from each substrate were prepared for experiment i.e. 100% pea residue (control); 90% pea
residue + 10% rice bran; 90% pea residue waste + 10% chicken manure; 100% maize stalk (control); 90% maize
stalk+ 10% rice bran; 90% maize stalk + 10% chicken manure; 100% banana leaves (control); 90% banana
leaves+ 10% rice bran, 90% banana leaves+ 10% chicken manure.