Visibly cloudy beers containing particles were first filtered through a paper filter; the other beers were used untreated. A
portion of 100 mL beer was transferred into a 250 mL Erlenmeyer flask and degassed by ultrasonication for 15 min. For analysis of ZEA, ergot alkaloids, and AOH, approximately 2 mL were pipetted into a test tube and the pH was adjusted to 7.0 ± 0.5 with aqueous Na2CO3 solution (1 mol/L, pH 12). Subsequently, the samples were diluted in enzyme immunoassay (EIA) working buffer solutions. For ZEA and ergot alkaloid analysis, sample dilutions and toxin standard
solutions were prepared in phosphate buffered saline (PBS; 0.01 mol/L) at a pH of 7.3 (ZEA) or 6.0 (ergot alkaloids), each containing 5% acetonitrile. For AOH analysis, samples were diluted in PBS (pH 7.3)
Visibly cloudy beers containing particles were first filtered through a paper filter; the other beers were used untreated. Aportion of 100 mL beer was transferred into a 250 mL Erlenmeyer flask and degassed by ultrasonication for 15 min. For analysis of ZEA, ergot alkaloids, and AOH, approximately 2 mL were pipetted into a test tube and the pH was adjusted to 7.0 ± 0.5 with aqueous Na2CO3 solution (1 mol/L, pH 12). Subsequently, the samples were diluted in enzyme immunoassay (EIA) working buffer solutions. For ZEA and ergot alkaloid analysis, sample dilutions and toxin standardsolutions were prepared in phosphate buffered saline (PBS; 0.01 mol/L) at a pH of 7.3 (ZEA) or 6.0 (ergot alkaloids), each containing 5% acetonitrile. For AOH analysis, samples were diluted in PBS (pH 7.3)
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