Tannins in the extract of high
molecular weight were precipitated using
protein from non-fat milk. The protein was
prepared by mixing fresh non-fat milk with
5% acetic acid (5:1, v/v).It was then held
at room temperature overnight, and then the
supernatant was discarded.The extracts with
high molecular weight tannin and protein
from non-fat milk were mixed, held at room
temperature overnight, and the upper solution
was collected.The pH of the extract
was adjusted to 6 by using 3M of NaOH,
rested at room temperature overnight,
and the upper solution was collected. The
polyphenols in the final solution were dried
using a spray drying method. Total phenolic
compounds in each gram of tamarind seed
coat extract powder were analyzed by using
the Folin-Ciocalteau method10.