Pairwise distance analysis of primer sequences (data not shown) against 15 animal and five plant species computed using the maximum composite likelihood method ( Tamura et al., 2011) revealed a minimum distance between monkey and pigeon (0.35) and maximum distance between rat and tuna (1.63) reflecting very high genetic distance and very little or no probability of cross-amplification of non-target species ( Ali et al., 2013). Construction of phylogenetic tree among the said species reveled similar outcomes (data not shown).