A streptomycin resistant E. coli strain HB101 was used in this
study. E. coli was grown overnight in Luria Bertani broth (Difco
Laboratories, Detroit, MI) supplemented with Streptomycin at 37 C
with constant agitation at 175 rpm. Cultures were washed by
centrifugation (4000 g, 15 min, 4 C) with 1 g L1 peptone saline
water, and added proportionally to distilled water to obtain an
initial inoculum solution. The concentration of E. coli cells in the
inoculation solution was diluted in order to reach a desired E. coli
inoculation of 105e106 CFU g1 on fresh-cut apples.