The ethanol fermentation process depends on many factors, such as initial glucose and inoculums concentrations, thetime required for efficient fermentation, reactor temperature, etc. The first set of experiments (first cycle) was conducted in order to compare the behavior of free and immobilized yeast. Cell count of pre-hydrated yeast, before polymerization tramped yielded a result of 3.6 × 105cell/ml. The initial glucose concentration was 30 g/l, the temperature was fixed at 42◦C and rotary shaker was set-up at 250 rpm. The fermentation was carried out during 90 h. Data of ethanol and glucose concentration sachieved at different operational time are reported in Table 4 and corresponding with the kinetics show in Fig. 5a and b.