The growth of each organism was analyzed by monitoring the cell division in presence of varying concentration of the dye. Samples of cultures were incubated at optimum temperature and periodically measured for cell density till stationary phase was reached. The OD (Optical Density) value was measured by Eppendorf Biophotometer in UVette having path length of 10 mm. Controls
were kept for each concentration tested and all tests were done in triplicates