The consumption of arabinose and fucose was negligible and it was also reported that Acetobacter xylinum strains have poor capability to utilize arabinose (Guo et al., 2013). Similar results were also reported by Cavka et al. (2013) and Guo et al.(2013) for production of bacterial cellulose from waste fiber sludge and detoxification of spruce hydrolysate for bacterial cellulose production,respectively. The analysis of the media before and after with Acetobacter xylinus 23769 indicates that the consumption of glucose and xylose were around 0.03 g/l and 0.16 g/l during 28 days of fermentation at pH 8 and 28◦C. The utilization of xylose by Acetobacter xylinum is not well understood but it has been proposed that xylose is mainly used as a source of energy for the bacterium, as it is consumed by Acetobacter xylinum through conversion into glyceraldehyde-3-phosphate,which in turn, ends up in the tricarboxylic acid cycle (TCA) as acetylCo-A after several other reactions (Ross, Mayer, & Benziman, 1991;Cavka et al., 2013). This study of fermentation media composed ofHWE further supports the suggestion that Acetobacter xylinum does consumexylose