The global population is increasing exponentially leading to increasing dependency on fossil fuel and imparting huge pressure on the energy crisis impeding sustainable development. This ever- increasing dependency on fossil fuels has contributed to climate change and global warming, thus leading to severe impact on the environment. In this current century there is a paradigm shift on reuse of waste and the research on conversion of waste to green energy. There is a renewed interest in the search for new, cleaner and green energy source. In the scope for search of new cleaner and sustainable energy, research efforts have mainly focused on energy carriers such as hydrogen, ethanol, butanol, biodiesel, and methane. More recently research has been also focused on biohythane and hythane, which are a mixture of hydrogen and methane [1] and [2]. Hythane®, a mixture of natural gas and hydrogen, usually has 5–7% hydrogen which makes it a high value gas fuel for vehicles and combustion engines [3]. Biohythane production technology is considered more energy-efficient because it involves anaerobic digestion which is a simpler process for conversion of acetate to methane after the acidogenic phase of hydrogen fermentation [4].