The mating types of 124 and 88 single spore isolates, isolated at random from Volvariella volvacea strains
V23 (A mating type locus alleles A1 + A2) and PY (A mating type locus alleles A3 + A4) respectively, were
determined by molecular screening using four primer pairs designed according to the sequences of the
A mating type genes (HD1 and HD2) of the two parental strains.