However, all species used here contained levels
of 20:5n-3 and 22:6n-3 that are typical for the species,
so it is possible that the formation of these fatty acids
from 18:3n-3 was repressed by a tissue PUFA or that
adequate PUFAs were obtained from the algal diet. The
experimental approach described here would
allow investigation of long chain PUFA synthesis in
zooplankton under different dietary regimes, particularly
for PUFA poor diets and at different developmental
stages.