Protein purification was
carried by the affinity chromatography protocol previous described
by Ramos and co-workers [47] using Sephadex G-50 column
(10 50 cm). The ConM was complexed with IAA in presence of
light for 30 min at 310 K and haemagglutinating activity (HA) was
done to confirming that indole binding does not alter the carbohydrate
recognition.