Centrifuge the tube at 2000 × g for 5 minutes at 4°C to pellet the DNA. 6. Remove the phenol-ethanol supernatant and save it in a new tube if protein isolation is desired. The supernatant can be stored at –70°C for several months
Centrifuge the tube at 2000 × g for 5 minutes at 4°C topellet the DNA.6. Remove the phenol-ethanol supernatant and save it in anew tube if protein isolation is desired. The supernatantcan be stored at –70°C for several months