2.2. Fruit material
Peach [Prunus persica (L.) Batsch cv. Baifeng] fruits were handharvested
at firm-mature stage from a commercial orchard in Nanjing,
China, and transported to the laboratory on the day of collection.
In the laboratory, the fruits were selected for uniform size and
maturity and absence of visual defects. Fruits were surface-sterilized
with 75% ethanol, and air dried prior to wounding.
2.3. Efficacy of B. cereus AR156 for control of Rhizopus rot
Peaches were wounded with the tip of a sterile dissecting needle
and two uniform 4 mm deep and 2 mm wide wounds made at
two sides of each fruit around the fruit equator. 20 ll of washedcell
suspension of B. cereus AR156 at 1 108 CFU ml1 or distilled
water (as control) was pipetted onto each wound. The fruit were
then air dried and put into 400 300 100 mm plastic trays
wrapped with high density polyethylene sleeve. After keeping at
20 C for 12 h, fruit pretreated with the biocontrol agent or distilled
water were inoculated with 15 ll of a suspension of 1 105 spores
of per ml R. stolonifer in each wound. The fruits were incubated at
20 C with high humidity (about 95%) for 3 days. Disease incidence
and lesion diameter on each fruit wound were observed at 1, 2 and
3 days postinoculation. Meanwhile, fruit samples were taken for
enzyme assays and measurements of protein, total phenolic and
H2O2 contents, DPPH radical-scavenging activity and quality
parameters. There were three replicate of 10 fruit each per treatment,
and the experiment was conducted three times.
2.2. Fruit material
Peach [Prunus persica (L.) Batsch cv. Baifeng] fruits were handharvested
at firm-mature stage from a commercial orchard in Nanjing,
China, and transported to the laboratory on the day of collection.
In the laboratory, the fruits were selected for uniform size and
maturity and absence of visual defects. Fruits were surface-sterilized
with 75% ethanol, and air dried prior to wounding.
2.3. Efficacy of B. cereus AR156 for control of Rhizopus rot
Peaches were wounded with the tip of a sterile dissecting needle
and two uniform 4 mm deep and 2 mm wide wounds made at
two sides of each fruit around the fruit equator. 20 ll of washedcell
suspension of B. cereus AR156 at 1 108 CFU ml1 or distilled
water (as control) was pipetted onto each wound. The fruit were
then air dried and put into 400 300 100 mm plastic trays
wrapped with high density polyethylene sleeve. After keeping at
20 C for 12 h, fruit pretreated with the biocontrol agent or distilled
water were inoculated with 15 ll of a suspension of 1 105 spores
of per ml R. stolonifer in each wound. The fruits were incubated at
20 C with high humidity (about 95%) for 3 days. Disease incidence
and lesion diameter on each fruit wound were observed at 1, 2 and
3 days postinoculation. Meanwhile, fruit samples were taken for
enzyme assays and measurements of protein, total phenolic and
H2O2 contents, DPPH radical-scavenging activity and quality
parameters. There were three replicate of 10 fruit each per treatment,
and the experiment was conducted three times.
การแปล กรุณารอสักครู่..