Main culture-dependent and independent methods used for bioprospection of microorganisms. From an environmental
sample, microorganisms can either be cultured using traditional or high-throughput methods (1). Alternatively, DNA can be
extracted directly from the sample (culture-independent methods, 2). DNA from pure cultures or the environmental sample can be
cloned to generate genomic or metagenomic libraries, respectively (3). These libraries can be screened by functional (4) or
molecular (5) methods to search for biomolecules of interest. Shotgun sequencing can aid in the design of molecular screening
methods