An extract of coconut milk lipase in acetone powder was prepared according to Hassanien and Mukherjee (1986), modified as follows.
A sample of 25 g of coconut milk was added to cold acetone at 4C and homogenized.
This crude extract was filtered through a cheese-cloth to remove precipitating material from the solution.
The residue was left to dry at room temperature to yield the acetone powder and left in the chiller at 4C till the assay was conducted.