methanol (0.5 ml), and 10 μl of this solution was applied
on to a thin-layer plastic plate coated with siliga gel
GF254 (Polygram® SIL G/UV254, 0.25 mm thickness,
20 cm x 20 cm). The TLC plate was then placed in a
chamber with chloroform and methanol (9:1) as mobile
phase. After development, the plate was removed, and
allowed to dry in air and examined under ultraviolet light
(254 nm and 366 nm). Then, the plate was sprayed with
vanillin-sulfuric acid reagent and heated in an oven at
120 °C for 10 minutes.