InordertomeasurethepHofthewashedresidues,25mLdistilledwaterwasaddedto2.5gofresiduesandafterreciprocatingshakeofthesamplesfor16h,thesuspensionswerefilteredandthepHofwashingswasmeasuredwithpHmeter[16].ThetotalconcentrationofCa,Mg,K,NaandPwasdeterminedusingdigestionbynitricacid.Groundplantmaterial(0.5g)ofeachresiduewasweighedindu-plicateintodigestiontubes.TenmLofconcentratednitricacidwasadded.Thetubeswerethenheatedto60°Cinthewaterbath.After3h,thetemperaturewasincreasedto95°Canddigestioncontin-uedforafurther6h.Thetubeswerethenallowedtocoolinroomtemperature.ThedigestswerefilteredandCaandMgcontentsoftheextractsweredeterminedbymethodofcomplexometrictitration,K
andNameasuredbyflameemissionspectrometry[16]andthetotalPcontentofdigestwasmeasuredbythemolybdenumbluemethod[17].ThetotalconcentrationofheavymetalswasdeterminedbythemodifiedmethodofCaoetal.[18]asfollows:groundplantsamples(0.2g)weretreatedwith4mLconcentratednitricacidduring1hin60°Cinwaterbathandthenthetemperatureincreasedto100°Candallowedtostayfor90min.Thenthesampleswereremovedfromwaterbathand0.2mLofhydrogenperoxidewasaddedtosam-plesandallowedtoremaininroomtemperaturefor30min.Thenthesampleswerefilteredandtheextractswerebroughttoavol-umeof25mLandthesolutionsamplesstored.Theconcentrationofheavymetalswasmeasuredbyatomicabsorptionspectrophotome-ter(AAS;Varian,spectra220).Alsotheair-dried,groundsamplesoftheorganicresidueswereanalyzedfortotalNbyKjeldahldigestionasdescribedbyRowell[19]andfororganicCbythelossonignitionprocedure[19].
InordertomeasurethepHofthewashedresidues, 25mLdistilledwaterwasaddedto2.5gofresiduesandafterreciprocatingshakeofthesamplesfor16h, thesuspensionswerefilteredandthepHofwashingswasmeasuredwithpHmeter [16] Ofeachresiduewasweighedindu-plicateintodigestiontubes ThetotalconcentrationofCa,Mg,K,NaandPwasdeterminedusingdigestionbynitricacid.Groundplantmaterial (0.5 กรัม) TenmLofconcentratednitricacidwasadded.Thetubeswerethenheatedto60°Cinthewaterbath.After3h,thetemperaturewasincreasedto95 ° Canddigestioncontin-uedforafurther6h.Thetubeswerethenallowedtocoolinroomtemperature.ThedigestswerefilteredandCaandMgcontentsoftheextractsweredeterminedbymethodofcomplexometrictitration,KandthetotalPcontentofdigestwasmeasuredbythemolybdenumbluemethod andNameasuredbyflameemissionspectrometry [16] [17] ThetotalconcentrationofheavymetalswasdeterminedbythemodifiedmethodofCaoetal [18] asfollows:groundplantsamples(0.2g) weretreatedwith4mLconcentratednitricacidduring1hin60 ° Cinwaterbathandthenthetemperatureincreasedto100 ° Candallowedtostayfor90min.Thenthesampleswereremovedfromwaterbathand0.2mLofhydrogenperoxidewasaddedtosam-plesandallowedtoremaininroomtemperaturefor30min.Thenthesampleswerefilteredandtheextractswerebroughttoavol-umeof25mLandthesolutionsamplesstored.Theconcentrationofheavymetalswasmeasuredbyatomicabsorptionspectrophotome-เธอ (AAS แล้วแต่กำหนด spectra220) Alsotheair แห้ง groundsamplesoftheorganicresidueswereanalyzedfortotalNbyKjeldahldigestionasdescribedbyRowell [19] andfororganicCbythelossonignitionprocedure [19]
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inordertomeasurethephofthewashedresidues 25mldistilledwaterwasaddedto2.5gofresiduesandafterreciprocatingshakeofthesamplesfor16h thesuspensionswerefilteredandthephofwashingswasmeasuredwithphmeter , , [ 16 ] thetotalconcentrationofca , Mg , K , naandpwasdeterminedusingdigestionbynitricacid . groundplantmaterial ( 0.5 g ) ofeachresiduewasweighedindu-plicateintodigestiontubes.tenmlofconcentratednitricacidwasadded .thetubeswerethenheatedto60 ° cinthewaterbath . after3h thetemperaturewasincreasedto95 องศา , canddigestioncontin-uedforafurther6h . thetubeswerethenallowedtocoolinroomtemperature . thedigestswerefilteredandcaandmgcontentsoftheextractsweredeterminedbymethodofcomplexometrictitration K
andnameasuredbyflameemissionspectrometry [ 16 ] andthetotalpcontentofdigestwasmeasuredbythemolybdenumbluemethod [ 17 ]thetotalconcentrationofheavymetalswasdeterminedbythemodifiedmethodofcaoetal . [ 18 ] ดังนี้ groundplantsamples ( 0.2g ) weretreatedwith4mlconcentratednitricacidduring1hin60 ° cinwaterbathandthenthetemperatureincreasedto100 ° candallowedtostayfor90min.thenthesampleswereremovedfromwaterbathand0.2mlofhydrogenperoxidewasaddedtosam-plesandallowedtoremaininroomtemperaturefor30min .thenthesampleswerefilteredandtheextractswerebroughttoavol-umeof25mlandthesolutionsamplesstored . theconcentrationofheavymetalswasmeasuredbyatomicabsorptionspectrophotome ตรี ( AAS ; เครื่อง spectra220 , ) alsotheair แห้ง groundsamplesoftheorganicresidueswereanalyzedfortotalnbykjeldahldigestionasdescribedbyrowell [ 19 ] andfororganiccbythelossonignitionprocedure [ 19 ]
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