Fruit juice drinks containing hydrolyzed collagen were formulated and produced as a new
functional drink from orange, apple and white grape juice blends containing ingredients
such as hydrolyzed fish collagen, citric acid, ascorbic acid, and natural mint flavour. Difference
and preference sensory tests were conducted to select the most preferred formulation
containing an addition of 1–3% hydrolyzed collagen. The results indicate that the formulation
with the addition of 2.5% hydrolyzed collagen was significantly preferred. Drinks at
pH 3.96–4.04 were pasteurized at 95 °C until reaching P value necessary for 3D decimal reduction
of Alicyclobacillus acidoterrestris. Addition of hydrolyzed collagen increased the protein
content of the drinks from 0.56 to 2.22–2.48 g/100 mL. The in vitro bioavailability results indicated
that the orange (95.37%) and apple (90.71%) drinks showed a higher bioavailability
(5–14%) than the white grape juice blends. The ascorbic acid content (81.39–113.5 mg/
100 mL), total phenolic content (86.93–117.43 mg GAE/100 mL), and antioxidant capacity ABTS
assay results (104–127 mol TEAC/100 mL) varied widely in the drinks.