Statistical analysis showed there was no significant effect of glyphosate on the relative abundances of seven functional microbial groups. Of these 126 nonparametric analyses of variance, only 2 had a significant effect at P < 0.05. These two significant results were in the Blount1 soil on Day 60, where Gm+ and total bacterial EL-FAMEs were less in both glyphosate treatments compared to the control.