DPPH radical scavenging activity assay
DPPH radical scavenging activity was assayed
according to Gupta and Prakash (2009). Different
concentrations of methanolic extract were taken in
different test tubes and the volume made to 1 mL with
methanol. 4 mL of 0.1 mM methanolic solution of
DPPH was added. The tubes were shaken vigorously
and allowed to stand for 15 minutes at room
temperature. A control was prepared as above without
any sample and methanol was used for base line
correction. Changes in absorbance of samples were
measured at 517 nm. DPPH free radical scavenging