Orange proteomic fingerprinting: From fruit to commercial juices
Combinatorial peptide ligand library technology, coupled to mass spectrometry, has been applied to
extensively map the proteome of orange pulp and peel and, via this fingerprinting, to detect its presence
in commercial orange juices and drinks. The native and denaturing extraction protocols have captured
1109 orange proteins, as identified by LC–MS/MS. This proteomic map has been searched in an orange
concentrate, from a Spanish juice manufacturer, as well as in commercial orange juices and soft drinks.
The presence of numerous orange proteins in commercial juices has demonstrated the genuineness of
these products, prepared by using orange fruits as original ingredients. However, the low number of
identified proteins in sparkling beverages has suggested that they were prepared with scarce amounts
of fruit extract, thus imparting lower quality to the final products. These findings not only increase the
knowledge of the orange proteome but also present a reliable analytical method to assess quality and
genuineness of commercial products.
Orange proteomic fingerprinting: From fruit to commercial juicesCombinatorial peptide ligand library technology, coupled to mass spectrometry, has been applied toextensively map the proteome of orange pulp and peel and, via this fingerprinting, to detect its presencein commercial orange juices and drinks. The native and denaturing extraction protocols have captured1109 orange proteins, as identified by LC–MS/MS. This proteomic map has been searched in an orangeconcentrate, from a Spanish juice manufacturer, as well as in commercial orange juices and soft drinks.The presence of numerous orange proteins in commercial juices has demonstrated the genuineness ofthese products, prepared by using orange fruits as original ingredients. However, the low number ofidentified proteins in sparkling beverages has suggested that they were prepared with scarce amountsof fruit extract, thus imparting lower quality to the final products. These findings not only increase theknowledge of the orange proteome but also present a reliable analytical method to assess quality andgenuineness of commercial products.
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