The TAS assay was carried out using Cayman’s Antioxidant Assay Kit. Into the each of the sample wells, 10 μL
of three series of concentrations including 500; 125; 31.25 μg/mL of GMRextract and fractions, 10 μL
metmyoglobin, and 150 μL chromogen were added. For Trolox standard wells, 10 μL trolox standard (tubes A-G),
metmyoglobin, and 150 μL chromogen were put.