E. intestinalis was collected from brackishwater
ponds around Iloilo, namely Arevalo, Duangas and Leganes. Seaweeds were transported
to the University multispecies Hatchery, Miagao, Iloilo and were manually washed with
freshwater, shade-dried for 3 to 4 days, ground using a miller, and kept frozen (-20 oC)
until use. The whole experiment was done from September 2012 to March 2013.