For the comparison of shoot induction
ability of different explants, the shoot tips,
nodal and internodal stem segments (1 cm),
and leaf segments were excised from the
previously micropropagated shoots bearing 3-
4 internodes and were inoculated on MS
basal media supplemented with 1.0 mg L-1 6-
benzyladenine (BA). To investigate the
effects of different PGRs on shoot
proliferation, nodal stem segments were
inoculated on MS basal media supplemented
with different concentrations (0.0, 0.5, 1.0,
2.0, and 4.0 mg L-1) of cytokinins BA, kinetin
(Kin) and thidiazuron (TDZ). All of the
cultures were subcultured onto the fresh
medium with the same composition at two
weeks intervals. The percentage of shoot
proliferation, number of shoots per explant,
and mean shoot length were recorded every
week for eight weeks.