2.2. Preparation of A. annua infusion
Fine powder from A. annua leaves was provided by Anamed,
Germany (batch no. 091002-DE) and kept in the original package
at room temperature protected from sunlight until use. During
each day of treatment, the Anamed recipe was followed by weigh-
ing 1 g of A. annua leaf powder in a 100-ml Erlenmeyer flask and
adding 100 ml of boiled water (Hirt, 2000). The infusion was then
covered with aluminum foil and left to cool for 15 min. The infu-
sion was then filtered and left to cool for a further 20 min. Samples
were prepared daily and stored at ambient temperature