A procedure for plant regeneration of cassava (Manihot esculenta) via somatic embryogenesis
from immature leaf explants cultured in vitro grown plants were developed. The explants
showed a differentiated the somatic embryos when cultured on Murashige and skoog medium
(MS) with 2% sucrose and the concentration of 2,4-D. The best media for induction of somatic
embryogenesis consisted of MS and 2% sucrose supplemented with 7 mg/l 2,4-D. After 10-15
days of culture, the somatic embryo germination varied between 13.6-92.6% and plant
conversion percentage between 17.9-42.6%. Whole plants were successfully transferred to soil.