The healthy rhizome was cut and removed from the
plant and fixed in FAA (formalin - 5ml + acetic acid -
5ml + 70% ethyl alcohol - 90ml). After 24 hrs of fixing,
the specimens were dehydrated as per schedule.
Infiltration of the specimens was carried by gradual
addition of paraffin wax (melting point 58-60°C) until
tertiary-butyl alcohol solution attained super saturation.
The specimens were cast into paraffin blocks. The
paraffin embedded specimens were sectioned with the
help of Rotary Microtome18. Dewaxing of the sections
was carried out by standard procedure and stained the
method published by O’Brien et al.,19. The photographs
were taken through the microscope.