The preliminary trial with shell with or without
membranes caused a substantial reduction in the BW
while ESM alone supported growth (not shown). Similarly, the relative weights of both heart and liver increased significantly in groups receiving feed with shell
containing preparations indicating their toxic effects.
The chickens receiving feed supplemented with ESM
only did not show any change in relative organ weights
(not shown). The results of the final trial are shown
later.
The analysis of ESM showed the nitrogen content
∼86% of total mass. When ESM added at 0.2 or 0.4%
levels to feed, showed negligible differences compared
with the total protein, caloric, or elemental content of
regular diet