3.2. In vitro cytotoxicity
Acrolein as positive control substance (data not shown) as well
as TPM and GVP of the reference cigarette 2R4F produced cytotoxicity
results consistent with historical data from the laboratory.
Cytotoxicity results on a per cigarette basis are given in the
Appendix in Table C. There were no significant differences between
the mean 1/EC50 values for Mixes A, B and C, compared with the
appropriate control cigarette data, for both the TPM and the GVP.
When compared on a per mg TPM basis, according to the nearly
identical TPM yield of the cigarettes, there were also no significant
differences between control and test cigarettes for both the TPM
and GVP fractions (Fig. 4). Cytotoxicity of TPM from the reference
cigarette 2R4F was approximately the same as that from the experimental
cigarettes (control and test), but the cytotoxicity of the
GVP was approximately twice as high.
3.2. In vitro cytotoxicity
Acrolein as positive control substance (data not shown) as well
as TPM and GVP of the reference cigarette 2R4F produced cytotoxicity
results consistent with historical data from the laboratory.
Cytotoxicity results on a per cigarette basis are given in the
Appendix in Table C. There were no significant differences between
the mean 1/EC50 values for Mixes A, B and C, compared with the
appropriate control cigarette data, for both the TPM and the GVP.
When compared on a per mg TPM basis, according to the nearly
identical TPM yield of the cigarettes, there were also no significant
differences between control and test cigarettes for both the TPM
and GVP fractions (Fig. 4). Cytotoxicity of TPM from the reference
cigarette 2R4F was approximately the same as that from the experimental
cigarettes (control and test), but the cytotoxicity of the
GVP was approximately twice as high.
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