The assays performed for each of the parameters under
investigation were carried out on 5e6 rats per group. The control
group received bacteria-free water. The body weights of the animals
were measured automatically after 30 days for each rat. The
rats were kept in well ventilated house conditions. A positive
control of rats receiving orlistat (F. Hoffmann-La Roche Ltd., Swiss
Health-Care Company, Basel, Switzerland) at 2 mg/kg body weight,
for 30 days was used.