2.3. Data collection and statistical analysis Ratio of germination and elongation were calculated as described in an earlier report [10]. RGR = GRT/GRC x 100 where, RGR= relative germination ratio, GRT = germination ratio of test plant and GRC = germination ratio of control plant. RER = MLT/ MLC x 100 where, RER= relative elongation ratio, MLT = mean length of test plant and MLC = mean length of control plant. For the calculation of percentage of inhibitory effect on the elongation, a previously described formula was used [11]: I= 100- (ET/EC x 100) where, I is the % inhibition, ET is the elongation of test plant and EC is the elongation of control plant. The average data obtained from four experimental replication were analysed using Graphpad prism 5 software (GraphPad Software, CA, USA). One-way and two-way analyses of variance (ANOVAs) were used to compare the differences between the control and the test plants. P < 0.05 was considered to be statistically significant.