2.5.3. Methanolic extracts (chemical extracts; CE)
Powdered samples (1 g) were extracted for 1 h with 25 mL of
methanol: water mixture (4:2, v/v). The extracts were separated by
decantation and the residues were extracted again with 25 mL of
methanol: water mixture (1:1, v/v). Extracts were combined and
stored in darkness at 20 C.