2.7. Microbiological analysis
The amount of 1.00 ± 0.01 g of mushroom sample was transferred
to a bottle containing 99 ml of sterile distilled water, and
vigorously shaken (30 s). Next, serial dilution of the samples from
104 to 106 were prepared in sterile distilled water, from which
cultures were carried out on the appropriate media. The yeasts and
molds number was determined according to PN-90/A-75052.08,
the coli index was evaluated in the LPB broth (with lactose and
bromocresol purple), and the number of lactic acid bacteria was
assessed according to PN-90/A-75052/07. The TVC (Total Viable
Count) and the amount of spore-forming bacteria (Bacillus) were
determined according to PN-EN ISO 4833:2004/Ap1:2005 by
culturing the sample diluted 1:1000000, respectively before and
after 15 min of boiling.
2.7. Microbiological analysis
The amount of 1.00 ± 0.01 g of mushroom sample was transferred
to a bottle containing 99 ml of sterile distilled water, and
vigorously shaken (30 s). Next, serial dilution of the samples from
104 to 106 were prepared in sterile distilled water, from which
cultures were carried out on the appropriate media. The yeasts and
molds number was determined according to PN-90/A-75052.08,
the coli index was evaluated in the LPB broth (with lactose and
bromocresol purple), and the number of lactic acid bacteria was
assessed according to PN-90/A-75052/07. The TVC (Total Viable
Count) and the amount of spore-forming bacteria (Bacillus) were
determined according to PN-EN ISO 4833:2004/Ap1:2005 by
culturing the sample diluted 1:1000000, respectively before and
after 15 min of boiling.
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