1. Biohydrogen was successfully produced from starch wastewater via an up-flow
anaerobic staged reactor (UASR).
2. Hydrogen yield (HY), hydrogen production rate (HPR), and specific HPR (SHPR)
peaked at OLR of 54 g-COD/L.d and F/M of 1.4 g-COD/g-VSS.d, resulting 1.87 mol-
H2/mol-glucose, 246 mmol-H2/L.d, and 145 mL-H2/g-VSS.d, respectively.
3. The initial cultivation pH and substrate concentration markedly affected hydrogenproducing
bioactivity.
4. Starch wastewater had an optimal initial cultivation pH of 5.5 with a maximum HPR
of 1.25 mmol-H2/L.h and initial substrate concentration of 20 g-starch/L with a
maximum specific HPR of 0.85 mmol-H2/g-VSS.d.
5. For all substrate concentrations, the 2nd order kinetic was effectively able to track the
measured data with R2> 0.97
CONCLUSIONS