Volatile Flavor Compounds
The volatile compounds of Cheddar cheeses with varying salt levels were isolated using a dynamic headspace analyzer coupled with a gas chromatograph (Agilent Technologies Ireland Ltd., Little Island, Co. Cork, Ireland). The samples were prepared using the procedure of Rulikowska et al. (2013). The trapped volatiles were injected to the column of the gas chromatograph equipped with a capillary column. Mean peak areas were calculated for individual compounds using the Chemstation software and standard library (Agilent Technologies Ireland Ltd.). Volatiles analyses were performed in triplicate after 180 d of ripening.