Spore suspensions (0.5 ml) of strain SM101 at an
OD600 w1 (w108 spores/ml) in 25 mM phosphate buffer (pH 7.0)
were placed in sterile plastic pouches and thermally sealed with an
impulse sealer (IPK-205HK, Impak Corp., Los Angeles, CA, USA).
Samples were treated with 100, 150 and 200 MPa for 7 min