Microwave-heate alkali pretreatment(MAP) was investigated to improve enzymatic digestiblity and production from water hyacinth.SEM revealed that MAP deconstructed the monopolies matrix and swelled the surfaces of water hyacinth.XRD indicated that:MAR decreased the crystalline index from 16.0 to 13.0 because of cellulose amorphseation.FTIR indicated that.MAP effective destroyed the lignin structure and disrupted the crystalline cellulose to reduce crystallinity. The reducing sugar yield of 0.296 g/gTVS was achieved at optimal hydrolysis conditions (microwave temperature=190 time=10 min, and celluose dosage=5at%).The sequentially fermentation hydrogen and methane yields from water hyacinth with MAP and enzymatic hydrolysis were increased to 63.9 and 172.5 ml./gTVS. respectively. The energy conversion efficiency(40.0%) in the two-stage hydrogen and methane regeneration was lower than that(49.5%) in the one stage methane production(237.4 mL./gTVS) from water hyacinth with MAP and enzymatic hydrolysis.